The anaerostat makes it possible to carry out a cultivation of microorganisms in an accurately defined protective atmosphere. Internal atmosphere can be created by:

• air expulsion with the help of the gas (for example from pressure cylinder)  that will create internal atmosphere inside anaerostat
• air aspiration of the anaerostat and subsequent filling with the required atmosphere
• use of anaerocult placed inside of the anaerostat (which creates the protective atmosphere throughout cultivation period inside the anaerostat)